Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3807 |
| PDBID: | 7JUM |
| Chains: | I_B |
| Organism: | Plasmodium falciparum 3D7, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1038/s41467-021-21955-1 |
| Antibody | |
| Antibody: | LMIV230-01 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Gametocyte surface protein P230 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: I
Mutation: NULL
| >7JUM_I|Chain D[auth H], E[auth I], F[auth J]|Neutralizing antibody, LMIV230-01 Single-chain Fv|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKVSGGTFNTYAIIWVRQAPGQGLEWMGAIIPFQDRGQYAQKFQGRVTITADKSTSTAYMELSSLRSEDTAVYYCAKESGRAVADRWGQGTLVTVSSGGGGSGGGGSGGGGSGGGGSGGGGSDIVMTQSPASLALSLGERATLYCRASHSISSWLAWYQQKPGKAPKLLIYKASTLESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQQYKSYPWTFGQGTKLEIKRHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >7JUM_B|Chain A, B, C|Gametocyte surface protein P230|Plasmodium falciparum (isolate 3D7) (36329) SVLQSGALPSVGVDELDKIDLSYETTESGDTAVSEDSYDKYASQNTNKEYVCDFTDQLKPTESGPKVKKCEVKVNEPLIKVKIICPLKGSVEKLYDNIEYVPKKSPYVVLTKEETKLKEKLLSKLIYGLLISPTVNEKENNFKEGVIEFTLPPVVHKATVFYFICDNSKTEDDNKKGNRGIVEVYVEPYGNKING |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: GLN1 VAL2 GLY26 GLY27 THR28 ASN30 THR31 TYR32 ALA33 ILE52 PHE54 GLN55 ARG57 GLN59 LYS74 LYS98 GLU99 SER100 GLY101 ARG102 ALA103 ARG107 SER173 TRP175 TYR192 LYS193 THR196 TYR234 LYS235 SER236 TYR237 TRP239 B: GLU565 THR566 THR567 GLU568 SER569 ASP571 ALA573 VAL574 SER575 GLU576 ASP577 TYR579 LYS581 LYS610 GLU612 LYS653 GLU655 THR656 LYS657 LYS659 LYS698 ALA699 THR700 VAL701 TYR703 GLU724 TYR726 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
