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Entry information

Complex
AACDB_ID: 3805
PDBID: 7JTR
Chains: H_G
Organism: Escherichia coli, Mus musculus
Method: XRD
Resolution (Å): 2.50
Reference: 10.1002/pro.4088
Antibody
Antibody: 7JTR scFv
Antibody mutation: No
INN (Clinical Trial):
Antigen
Antigen: Maltose/maltodextrin-binding periplasmic protein(MBP)
Antigen mutation: No
Durg Target: P0AEX9

Sequence information

Antibody

Chain: H
Mutation: NULL

>7JTR_H|Chain B, D, F, H|single-chain Fv antibody fragment (scFv)|Mus musculus (10090)
MQVQLQQPGSELVRPGASVKLSCKASGYTFTNSWMHWVKQRPGQGLEWIGNIFPGNGNTNYDENFKSKATLTVDTSSNTAYMQLSSLTSEDSAVYYCTRGLFAYWGQGTLVTVSAGGGGSGGGGSGGGGSDIVMTQSPSSLAMSVGQKVTMSCKSSQSLLINNNQKNYLAWYQQKPGQSPKLLVYFASTRESGVPDRFIGSGSGTDFTLTISSVQAEDLADYFCQQHFTAPYTFGGGTKLEIKREHHHHHH

Antigen

Chain: G
Mutation: NULL

>7JTR_G|Chain A, C, E, G|Maltose/maltodextrin-binding periplasmic protein|Escherichia coli (562)
KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRIT

Interaction

1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.

Interacting residues (ΔSASA based)

H: THR28 THR30 ASN31 SER32 TRP33 PHE52 ASN55 ASN57 ARG98 GLY99 LEU100 ALA102 LYS165 TYR167 LEU181 TYR184 PHE185 THR188 GLU190 SER191

G: GLU4 GLU22 LYS25 LYS26 PHE27 GLU28 LYS29 ASP30 THR31 GLY32 ILE33 LYS34 VAL35 GLU274 LEU275 GLU278 ASN282 TYR283

2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.

Interacting residues (Atom distance based)

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