Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3802 |
| PDBID: | 7JTR |
| Chains: | B_A |
| Organism: | Escherichia coli, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1002/pro.4088 |
| Antibody | |
| Antibody: | 7JTR scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Maltose/maltodextrin-binding periplasmic protein(MBP) |
| Antigen mutation: | No |
| Durg Target: | P0AEX9 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7JTR_B|Chain B, D, F, H|single-chain Fv antibody fragment (scFv)|Mus musculus (10090) MQVQLQQPGSELVRPGASVKLSCKASGYTFTNSWMHWVKQRPGQGLEWIGNIFPGNGNTNYDENFKSKATLTVDTSSNTAYMQLSSLTSEDSAVYYCTRGLFAYWGQGTLVTVSAGGGGSGGGGSGGGGSDIVMTQSPSSLAMSVGQKVTMSCKSSQSLLINNNQKNYLAWYQQKPGQSPKLLVYFASTRESGVPDRFIGSGSGTDFTLTISSVQAEDLADYFCQQHFTAPYTFGGGTKLEIKREHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7JTR_A|Chain A, C, E, G|Maltose/maltodextrin-binding periplasmic protein|Escherichia coli (562) KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRIT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR28 ASN31 SER32 TRP33 PHE52 ASN55 ASN57 ARG98 GLY99 LEU100 ALA102 TYR103 ILE160 GLN164 LYS165 TYR167 LEU181 TYR184 PHE185 THR188 GLU190 SER191 A: GLU4 ALA21 GLU22 LYS25 LYS26 GLU28 LYS29 ASP30 THR31 GLY32 ILE33 LYS34 VAL35 THR36 GLU274 LEU275 GLU278 ASN282 TYR283 GLU288 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
