Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3801 |
| PDBID: | 7JTI |
| Chains: | HL_A |
| Organism: | Mus musculus, Bos taurus |
| Method: | EM |
| Resolution (Å): | 7.40 |
| Reference: | 10.1096/fj.202000796RR |
| Antibody | |
| Antibody: | mAb5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interphotoreceptor retinoid-binding protein (IRBP) 3 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7JTI_H|Chain B[auth H]|mAb5 Fab heavy chain|Mus musculus (10090) QVQLKESGPGLVAPSQSLSITCTVSGFLLISNGVHWVRQPPGKGLEWLGVIWAGGNTNYNSALMSRVSISKDNSKSQVFLKMKSLQTDDTAMYYCARDFYDYDVFYYAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVP |
Light Chain: L
Mutation: NULL
| >7JTI_L|Chain A[auth L]|mAb5 Fab light chain|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLS |
Antigen
Chain: A
Mutation: NULL
| >7JTI_A|Chain C[auth A]|Retinol-binding protein 3|Bos taurus (9913) VIQRLQEALREYYTLVDRVPALLSHLAAMDLSSVVSEDDLVTKLNAGLQAVSEDPRLQVQVVRPKEASSGPEEEAEEPPEAVPEVPEDEAVRRALVDSVFQVSVLPGNVGYLRFDSFADASVLEVLGPYILHQVWEPLQDTEHLIMDLRQNPGGPSSAVPLLLSYFQSPDASPVRLFSTYDRRTNITREHFSQTELLGRPYGTQRGVYLLTSHRTATAAEELAFLMQSLGWATLVGEITAGSLLHTHTVSLLETPEGGLALTVPVLTFIDNHGECWLGGGVVPDAIVLAEEALDRAQEVLEFH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: THR30 THR31 SER32 ASN33 TYR34 GLY52 TRP93 TYR94 SER95 ASN96 H: TRP52 ALA53 GLY54 ASN56 ASN58 TYR100 ASP101 TYR102 ASP103 VAL104 PHE105 TYR106 TYR107 A: PRO83 PRO84 GLU85 GLU89 VAL90 PRO91 GLU92 ASP93 GLU94 ALA95 VAL96 ARG98 ALA99 LEU100 ASP102 SER103 GLN106 HIS137 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
