Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 38 |
| PDBID: | 1FJ1 |
| Chains: | DC_E |
| Organism: | Mus musculus, Borreliella burgdorferi B31 |
| Method: | XRD |
| Resolution (Å): | 2.68 |
| Reference: | 10.1006/jmbi.2000.4119 |
| Antibody | |
| Antibody: | LA-2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Outer surface protein A (OspA) of the Borrelia burgdorferi spirochete |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >1FJ1_D|Chain B, D|HYBRIDOMA ANTIBODY LA2 (HEAVY CHAIN)|Mus musculus (10090) QIQLVQSGPELKKPGETVKISCKASGYTFTDYSMYWVKQAPGKGLKRMGWINTETGEPTYADDFKGRFALSLDTSASTAYLHISNLKNEDTATYFCARGLDSWGQGTSVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPS |
Light Chain: C
Mutation: NULL
| >1FJ1_C|Chain A, C|HYBRIDOMA ANTIBODY LA2 (LIGHT CHAIN)|Mus musculus (10090) DIQMTQSPSSLSATLGGKVTITCKASQDINKYIAWYQHKPGKGPRLLIHYTSTLQPGNPSRFSGSGSGRDYSFSISNLEAEDIAIYYCLQYDNLQRTFGGGTKVEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: E
Mutation: NULL
| >1FJ1_E|Chain E, F|OUTER SURFACE PROTEIN A|Borrelia burgdorferi (224326) AKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSGVLEGVKADKCKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEITKLDEIKNALK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASN30 TYR32 LEU46 HIS49 TYR50 THR53 LEU54 GLN55 PRO56 TYR91 ASP92 LEU94 ARG96 D: ILE2 THR30 ASP31 TYR32 SER33 TRP50 ASN52 THR53 GLU54 ARG98 GLY99 LEU100 ASP101 E: LEU201 ASN202 SER206 SER207 ALA208 ALA209 LYS211 THR213 ALA214 ALA215 TRP216 THR226 VAL227 ASN228 SER229 LYS230 LYS231 ASP249 SER250 ASN251 GLY252 THR253 GLU256 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
