Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3797 |
| PDBID: | 7JR7 |
| Chains: | EF_B |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s42003-021-02039-8 |
| Antibody | |
| Antibody: | 2E10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | ATP-binding cassette sub-family G member 5 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7JR7_E|Chain E|Fab 2E10 heavy chain|Homo sapiens (9606) QVHLQQSGAELVRPGTSVKMSCKAAGYTFTYWWIGWLKQRPGHGLEWIGDFFPVSGHTNFNEKFRDKATLTADTSSSTAYMQLNSLTSEDSAIYYCARKQDSSGPLYAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: F
Mutation: NULL
| >7JR7_F|Chain F|Fab 2E10 light chain|Homo sapiens (9606) DIQMTQSSSYLSVSLGGRVTITCKASDHINNWLAWYQQKPGNAPRLLISAATSLETGVPSRFSGSGSGKDYTLSIISLQTEDVATYYCQQYWSTPFTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSTTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRA |
Antigen
Chain: B
Mutation: NULL
| >7JR7_B|Chain B|ATP-binding cassette sub-family G member 8|Homo sapiens (9606) MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: THR28 TYR31 TRP33 PHE52 VAL54 SER55 GLY56 HIS57 ASN59 PHE60 ARG65 ASP101 SER102 SER103 GLY104 PRO105 LEU106 F: ASN30 TRP32 TRP92 SER93 THR94 B: ASP33 ASN34 SER35 LEU36 TYR37 PHE38 THR39 TYR40 SER41 GLY42 GLN43 PRO44 ARG164 LYS186 ARG187 GLU189 ASP190 ALA193 ARG198 ASN230 LYS260 GLY261 ASN262 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
