Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3789 |
| PDBID: | 7JLK |
| Chains: | HL_B |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.99 |
| Reference: | 10.1016/j.celrep.2021.109084 |
| Antibody | |
| Antibody: | anti-idiotype iv1 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | glVRC01 Fab light chain |
| Antigen mutation: | No |
| Durg Target: | P01834 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7JLK_H|Chain A[auth H]|iv1 Heavy Chain|Mus musculus (10090) QIQLVQSGPELKKPGETVKISCKASGYTFTNYEINWVQQAPGKGLKLMGWINTYTGEPTSADDFKGRFAFSLETSASTAYLQINNLKNEDAATYFCARGDYYGSSSAWLPYWGQGTLVTVSAGSGSGHHHHHH |
Light Chain: L
Mutation: NULL
| >7JLK_L|Chain D[auth L]|iv1 Light Chain|Mus musculus (10090) DIVMTQSQKFMSTSVGDRVSITCKASQNVRTAVAWYQQKPGQSPKALIFLASNRHTGVPDRFTGSGSGTDFTLTISNVQSEDLADYFCLQHWNYPLTFGGGTKLEIKGGGGSGGGGSGGGGS |
Antigen
Chain: B
Mutation: NULL
| >7JLK_B|Chain C[auth B]|glVRC01 Light Chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQYEFFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: THR28 THR30 ASN31 TYR32 GLU33 THR52 TYR53 THR54 TYR97 GLY99 B: SER28 VAL29 SER30 SER31 TYR32 ASP50 ALA51 ASN53 GLY66 SER67 GLY68 PHE71 TYR91 GLU96 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
