Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3783 |
| PDBID: | 7JIE |
| Chains: | CD_B |
| Organism: | Norovirus Hu/GII.4/Sydney/NSW0514/2012/AU, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.25 |
| Reference: | 10.1038/s41467-021-24649-w |
| Antibody | |
| Antibody: | NORO-320 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human norovirus (HuNoV) GII.4 P-domain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >7JIE_C|Chain C, E|IgA Fab Heavy Chain|Homo sapiens (9606) QVQLVQSGPEVKKPGSSVKVSCKASGGTVSSYAISWVRQAPGQGLEWMGGIIPIFDTTNYAQKFQGRVTITADESTGTSDMELSSLRSEDTAVYYCARDRVPSYSPSRRFSTKGAMWGKYGMDVWGQGTLVTVSSASFKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: D
Mutation: NULL
| >7JIE_D|Chain D, F|IgA Fab Light Chain|Homo sapiens (9606) DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQLLIYLGSNRASGVPDRFSGSGSGTDFTLKISKVEAEDVGVYYCMQALQTPRTFGQGTKVEIKRTAAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHEGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7JIE_B|Chain A, B|VP1|Norovirus Hu/GII.4/Sydney/NSW0514/2012/AU (1241973) KPFSVPVLTVEEMTNSRFPIPLEKLFTGPSSAFVVQPQNGRCTTDGVLLGTTQLSPVNICTFRGDVTHITGSRNYTMNLASQNWNDYDPTEEIPAPLGTPDFVGKIQGVLTQTTRTDGSTRGHKATVYTGSADFAPKLGRVQFETDTDRDFEANQNTKFTPVGVIQDGGTTHRNEPQQWVLPSYSGRNTHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMDLDCLLPQEWVQYFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGQHDLVIPPNGYFRFDSWVNQFYTLAPMG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLN1 SER31 TYR32 ARG98 ARG100 VAL101 PRO102 TYR104 SER105 PRO106 SER107 LYS119 TYR120 ASP123 D: HIS31 ASN33 TYR35 TYR37 TYR54 LEU55 ASN58 SER61 B: LEU272 LEU278 ASN302 SER305 ASN307 ASN309 ASP310 TYR311 ASP312 PRO313 THR314 GLU315 GLU316 LEU321 ARG339 LYS361 LEU362 HIS417 TYR462 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
