Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3782 |
| PDBID: | 7JIC |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.80 |
| Reference: | 10.1126/science.abd9836 |
| Antibody | |
| Antibody: | Coltuximab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | B-lymphocyte antigen CD19 |
| Antigen mutation: | No |
| Durg Target: | P15391 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7JIC_H|Chain C[auth H]|Coltuximab Heavy Chain|Mus musculus (10090) QVQLVQPGAEVVKPGASVKLSCKTSGYTFTSNWMHWVKQAPGQGLEWIGEIDPSDSYTNYNQNFQGKAKLTVDKSTSTAYMEVSSLRSDDTAVYYCARGSNPYYYAMDYWGQGTSVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCGGSGLEVLFQ |
Light Chain: L
Mutation: NULL
| >7JIC_L|Chain D[auth L]|Coltuximab Light Chain|Mus musculus (10090) EIVLTQSPAIMSASPGERVTMTCSASSGVNYMHWYQQKPGTSPRRWIYDTSKLASGVPARFSGSGSGTDYSLTISSMEPEDAATYYCHQRGSXXYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7JIC_A|Chain A|B-lymphocyte antigen CD19|Homo sapiens (9606) DYKDDDDLEVLFQGPPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: THR30 SER31 ASN32 TRP33 SER53 ASP54 TYR56 ASN58 GLY98 SER99 ASN100 PRO101 TYR102 TYR103 TYR104 L: SER27 GLY28 ASN30 TYR31 ASP49 ARG90 A: GLY67 LEU68 GLN90 MET91 LYS105 TYR157 LYS161 ASP162 ARG163 PRO164 GLU165 ILE166 VAL217 HIS218 PRO219 LYS220 GLY221 PRO222 LYS223 SER224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
