Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3781 |
| PDBID: | 7JHH |
| Chains: | HL_A |
| Organism: | Homo sapiens, Escherichia coli K-12, synthetic construct |
| Method: | EM |
| Resolution (Å): | 3.92 |
| Reference: | 10.1126/science.abe7565 |
| Antibody | |
| Antibody: | Fab13 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 5'-AMP-activated protein kinase catalytic subunit alpha-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7JHH_H|Chain F[auth H]|Fab heavy chain|synthetic construct (32630) EISEVQLVESGGGLVQPGGSLRLSCAASGFNIYYYSIHWVRQAPGKGLEWVASIYPYSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYYPYFISYYSKMEAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >7JHH_L|Chain E[auth L]|Fab light chain|synthetic construct (32630) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSSGPITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7JHH_A|Chain A|5'-AMP-activated protein kinase catalytic subunit alpha-1|Homo sapiens (9606) VKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDELTPRPGSHTIEFFEMCANLIKILAQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER31 SER32 TYR50 SER53 TYR56 SER57 GLY58 ARG67 H: GLU4 VAL5 ALA27 SER28 GLY29 PHE30 ASN31 TYR33 TYR34 TYR35 TYR55 TYR57 SER58 THR77 LYS79 ASN80 ARG101 TYR103 PRO104 TYR105 PHE106 ILE107 SER108 TYR109 TYR110 LYS112 MET113 ASP117 TYR118 A: ARG55 LEU57 LYS62 ARG65 GLU66 ASN69 LEU70 PHE73 ASP130 HIS133 ARG134 HIS135 MET136 VAL137 VAL138 ARG140 GLY161 ASN164 MET165 ASP168 SER188 ARG190 LEU191 TYR192 ALA193 GLY194 PRO195 GLU196 ILE199 ASP254 PRO255 MET256 ARG258 ALA259 THR260 ILE261 LYS262 ARG265 SER284 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
