Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3777 |
| PDBID: | 7FJS |
| Chains: | HL_E |
| Organism: | Homo sapiens, Severe acute respiratory syndrome coronavirus 2 |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1016/j.isci.2022.104043 |
| Antibody | |
| Antibody: | T6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 B.1.351 spike glycoprotein RBD |
| Antigen mutation: | Yes |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7FJS_H|Chain C, F[auth H]|T6 heavy chain|Homo sapiens (9606) QVQLQQPGTELVNPGASLKMSCKTSGYRFTSYIIHWVKQTPGQGLEWIGAIFPENDDTSYSQKFKGKATLTTDTSSSTAYMQLSSLTSEDSAVYYCARDGENVLDYWGQGTSVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVE |
Light Chain: L
Mutation: NULL
| >7FJS_L|Chain A, D[auth L]|T6 light chain|Homo sapiens (9606) RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECQIVLTQSPSSLAVSVGEKVTLSCKSSQSLLYSNNQKNYLAWYQQKSGRSPKLLLHWTSTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYYTYPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: K85N/E152K/N169Y
| >7FJS_E|Chain B, E|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR31 ASN33 TYR38 TYR97 TYR98 THR99 TYR100 TRP102 H: SER31 TYR32 ILE33 ALA50 ILE51 PHE52 GLU54 ASN55 ASP57 THR58 SER59 ASP99 GLY100 GLU101 VAL103 E: TYR421 LEU455 PHE456 TYR473 GLN474 ALA475 GLY476 SER477 THR478 PRO479 ASN481 GLY485 PHE486 ASN487 TYR489 GLN493 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
