Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3769 |
| PDBID: | 7FIM |
| Chains: | N_B |
| Organism: | synthetic construct, Rattus norvegicus, Bos taurus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/s41467-022-28683-0 |
| Antibody | |
| Antibody: | 35 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7FIM_N|Chain A[auth N]|Nanobody-35|synthetic construct (32630) MAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: B
Mutation: S54N/G226A/E268A/N271K/K274D/R280K/T284D/I285T/A366S
| >7FIM_B|Chain B|Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1|Rattus norvegicus (10116) MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNVSGWRLFKKIS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: GLN3 VAL4 GLN5 GLY28 PHE29 TYR34 ARG100 PRO102 ALA103 PRO104 PHE105 THR116 ALA118 TYR119 GLN122 B: ARG13 GLU17 LYS20 THR169 THR189 GLY190 CYS209 ASP210 ALA211 THR228 GLY229 HIS230 GLU231 SER232 ASP233 ASP251 ASP252 ILE275 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
