Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3768 |
| PDBID: | 7FIM |
| Chains: | N_A |
| Organism: | synthetic construct, Rattus norvegicus, Bos taurus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/s41467-022-28683-0 |
| Antibody | |
| Antibody: | 35 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short |
| Antigen mutation: | No |
| Durg Target: | P63092 |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7FIM_N|Chain A[auth N]|Nanobody-35|synthetic construct (32630) MAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7FIM_A|Chain C[auth A]|Guanine nucleotide-binding protein G(s) subunit alpha isoforms short|Bos taurus (9913) MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: GLU48 TRP49 ASP52 SER61 TYR62 THR63 GLY64 SER65 LYS67 PRO102 THR106 ARG107 ASP108 CYS109 PHE110 ASP111 THR113 SER114 THR115 THR116 TYR117 ALA118 TYR119 A: ARG228 ASP229 GLU230 ARG231 ARG232 ILE235 ASN264 GLN267 ALA268 LYS271 LEU272 ASP274 SER275 ILE276 ASN278 ASN279 LYS280 ARG283 ASP310 TYR311 PHE312 PRO313 GLU314 SER352 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
