Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 376 |
| PDBID: | 2WUC |
| Chains: | HL_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.str.2009.09.014 |
| Antibody | |
| Antibody: | 40.deltaTrp Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | hepatocyte growth factor activator(HGFA) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2WUC_H|Chain C[auth H]|FAB FRAGMENT FAB40.DELTATRP HEAVY CHAIN|HOMO SAPIENS (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTINGTYIHWVRQAPGKGLEWVGGIYPAGGATYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCAKWAWPAFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: L
Mutation: NULL
| >2WUC_L|Chain E[auth L]|FAB FRAGMENT FAB40.DELTATRP LIGHT CHAIN|HOMO SAPIENS (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSNRAPATFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >2WUC_A|Chain A|HEPATOCYTE GROWTH FACTOR ACTIVATOR LONG CHAIN|HOMO SAPIENS (9606) IIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGVYTRVANYVDWINDRIRPPRRLVAPSAAAHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN30 GLY31 TYR33 HIS35 TRP47 PRO52 ALA53 GLY54 GLY55 ALA56 THR57 TYR58 LYS64 TRP95 ALA97 TRP98 PRO99 L: ASP28 SER30 ALA32 SER50 SER91 ASN92 ARG93 ALA94 ALA96 A: PHE59 ARG61 LYS87 TYR88 ILE89 PRO90 TYR91 THR92 LEU93 TYR94 SER95 VAL96 PHE97 ASN98 ASP100 HIS101 ILE176 SER177 PRO178 ASN179 ASN233 ASP236 TRP237 ASP240 ARG241 ARG243 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
