Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3758 |
| PDBID: | 7FCI |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s41586-022-04857-0 |
| Antibody | |
| Antibody: | YN69083 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human sodium taurocholate co-transporting polypeptide (NTCP) |
| Antigen mutation: | No |
| Durg Target: | Q14973 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7FCI_H|Chain C[auth H]|Fab Heavy chain|Mus musculus (10090) EVQLQQPGAELVKPGASVKLSCKTSGYTFTNYWMKWVKQRPGQGLEWIGEINPSNGGTNYNGKFKSKASLTVDKSSSTAYMQLSSLTSEDSAVYYCTILVYDAYYVFAMDYWGLGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLT |
Light Chain: L
Mutation: NULL
| >7FCI_L|Chain B[auth L]|Fab Light chain|Mus musculus (10090) DIVMTQSPAIMSASPGQKVTITCSASSSVNYMHWYQQKLGSSPKLWIYDTSKLALGVPARFSGSGSGTSYSLTISSMEAEDAASYFCHQWSSYPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >7FCI_A|Chain A|Sodium/bile acid cotransporter|Homo sapiens (9606) GPGAPMEAHNASAPFNFTLPPNFGKRPTDLALSVILVFMLFFIMLSLGCTMEFSKIKAHLWKPKGLAIALVAQYGIMPLTAFVLGKVFRLKNIEALAILVCGCSPGGNLSNVFSLAMKGDMNLSIVMTTCSTFCALGMMPLLLYIYSRGIYDGDLKDKVPYKGIVISLVLVLIPCTIGIVLKSKRPQYMRYVIKGGMIIILLCSVAVTVLSAINVGKSIMFAMTPLLIATSSLMPFIGFLLGYVLSALFCLNGRCRRTVSMETGCQNVQLCSTILNVAFPPEVIGPLFFFPLLYMIFQLGEGLLLIAIFWCYEKFKTPKDKTKMIYTAATTEETIPGALGNGTYKGEDCSPCTA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ILE2 SER27 SER28 ASN30 TYR31 ASP49 LYS52 LEU55 TRP90 SER91 SER92 TYR93 ARG95 H: GLU1 VAL2 TYR27 ASN31 TYR32 TRP33 LYS35 GLU50 ASN52 ASN55 GLY57 THR58 ASN59 GLY62 LYS65 LEU99 VAL100 TYR101 ASP102 ALA103 TYR104 TYR105 VAL106 PHE107 MET109 TYR111 A: LEU14 ASN17 PHE18 GLY19 LYS20 PRO22 THR23 ASP24 LYS86 ASN87 ILE88 GLU89 TYR146 ASP147 GLY148 ASP152 LYS153 ILE208 ASN209 LYS212 PHE216 ALA217 THR219 ASN271 VAL272 ALA273 PHE274 PRO275 PRO276 GLU277 VAL278 GLY280 PRO281 PHE283 PHE284 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
