Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3745 |
| PDBID: | 7FAH |
| Chains: | CD_B |
| Organism: | Influenza A virus (A/California/04/2009(H1N1)), Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.15 |
| Reference: | 10.1038/s41467-022-32926-5 |
| Antibody | |
| Antibody: | 12H5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >7FAH_C|Chain B[auth C], E[auth H]|heavy chain of antibody 12H5|Mus musculus (10090) QIQLVQSGPELKKPGETVRISCKASGYTFTKNGMNWVQQAPGKGLKWVGWINTYTGEPSYADDFKGRFAFSLETSASTAYLQINNLKNEDMAAYFCARMVRDAMDFWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: D
Mutation: NULL
| >7FAH_D|Chain C[auth D], F[auth L]|Light chain of antibody 12H5|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCKASQSVDFDGYNYLNWYQQKPGQPPKLLIYAASNLESGIPARFSGSGSGTDFTLNIHPVEEEDAATYFCQQSNEDPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >7FAH_B|Chain A, D[auth B]|Hemagglutinin|Influenza A virus (strain swl A/California/04/2009 H1N1) (641501) MDTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADTYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREEIDSGRLVPRGSPGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: LYS31 ASN32 GLY33 TRP50 ASN52 TYR54 THR55 MET99 VAL100 ARG101 ASP102 D: PHE31 ASP32 GLY33 TYR34 TYR36 ASN57 SER95 ASN96 GLU97 TYR100 B: LYS63 GLU75 GLU77 LEU79 TYR100 LYS139 VAL141 THR142 ALA143 PRO146 HIS147 ALA148 GLY149 ALA150 LYS151 TRP159 VAL161 HIS189 ASP196 LEU200 ASP231 GLN232 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
