Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3737 |
| PDBID: | 7F9W |
| Chains: | BC_A |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/s41598-021-02449-y |
| Antibody | |
| Antibody: | CD25‑IL‑2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-2 receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | P01589 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7F9W_B|Chain C[auth B]|Heavy chain of Fab|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSDAINWVRQAPGQGLEWMGRIIPIFGVADYAQKFQGRVTLTADKSTSTAYMDLSSLRSEDTAVFYCARERGDYSNFWYFDLWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
Light Chain: C
Mutation: NULL
| >7F9W_C|Chain B[auth C]|Light chain of Fab|Homo sapiens (9606) DIQMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7F9W_A|Chain A|Interleukin-2 receptor subunit alpha|Homo sapiens (9606) DDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TYR31 SER33 ASN34 LYS36 TYR38 TRP56 TYR98 SER99 B: SER30 SER31 ASP32 ALA33 ARG50 ILE52 ILE54 PHE55 GLU99 ARG100 GLY101 ASP102 TYR103 SER104 ASN105 PHE106 TRP107 A: TYR128 TYR129 GLN130 CYS131 GLN133 GLY134 TYR135 ARG136 ALA137 LEU138 HIS139 ARG140 GLY141 PRO142 ALA143 GLU144 LEU161 THR164 GLY165 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
