Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3736 |
| PDBID: | 7F7E |
| Chains: | CL_E |
| Organism: | Homo sapiens, Severe acute respiratory syndrome coronavirus 2 |
| Method: | XRD |
| Resolution (Å): | 2.49 |
| Reference: | 10.1080/22221751.2022.2032374 |
| Antibody | |
| Antibody: | A5-10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein S1 |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >7F7E_C|Chain A[auth C]|Heavy chain of A5-10 Fab|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAIVGSGGSTYYADSVKGRFIISRDNSKNTLYLQMNSLRAEDTAVYYCAKSLIYGHYDILTGAYYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: L
Mutation: NULL
| >7F7E_L|Chain B[auth L]|Light chain of A5-10 Fab|Homo sapiens (9606) AQMTQSPSSVSASVGDRVTIPCRASQGIGNWLAWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPPFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTK |
Antigen
Chain: E
Mutation: NULL
| >7F7E_E|Chain C[auth E]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) PTNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER68 ILE115 TYR116 GLY117 HIS118 TYR119 ASP120 LEU122 THR123 GLY124 ALA125 TYR127 L: MET4 GLN27 GLY28 ILE29 GLY30 ASN31 TRP32 GLN90 ASN92 SER93 E: ASN343 THR345 ARG346 ASN439 ASN440 LEU441 ASP442 SER443 LYS444 VAL445 ASN448 ASN450 TYR451 PRO499 THR500 ARG509 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
