Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3713 |
| PDBID: | 7EO0 |
| Chains: | HL_3 |
| Organism: | Foot-and-mouth disease virus, Bos taurus |
| Method: | EM |
| Resolution (Å): | 3.75 |
| Reference: | 10.1128/JVI.00881-21 |
| Antibody | |
| Antibody: | C4 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Foot-and-mouth disease virus (FMDV) O/TIBET/99 VP3 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7EO0_H|Chain E[auth H]|Ig heavy chain variable region|Bos taurus (9913) QVQLRESGPSLVKPSQTLFLTCTVSGFSLTSYSVNWVRQTPGKMLECLGGIATSGSTGYNPVLKSRLRITKDNSKSQVSLSVSNVTPEDTATYYCAKWSSRGGYDCGVHSSDYSYLDAWGQGLLVTVSS |
Light Chain: L
Mutation: NULL
| >7EO0_L|Chain F[auth L]|Ig lamda chain variable region|Bos taurus (9913) WAQAVLTQPSSVSASLGQRVSITCSGSSSNIGRYGATWYQQVPGSGLRTIIYGSSRRPSGVPDRFSGSKSGNTVTLTISSLQPEDEADYFCAAYDISTNAVFGSGTTLTLLGDYKDDDDKGG |
Antigen
Chain: 3
Mutation: NULL
| >7EO0_3|Chain C[auth 3]|O/TIBET/99 VP3|Foot-and-mouth disease virus (12110) GIFPVACSDGYGGLVTTDPKTADPAYGKVFNPPRNMLPGRFTNFLDVAEACPTFLHFEGDVPYVTTKTDSDRVLAQFDLSLAAKHMSNTFLAGLAQYYTQYSGTINLHFMFTGPTDAKARYMIAYAPPGMEPPKTPEAAAHCIHAEWDTGLNSKFTFSIPYLSAADYAYTASDAAETTNVQGWVCLFQITHGKADGDALVVLASAGKDFELRLPVDARTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ALA52 THR53 SER54 GLY55 SER56 THR57 GLY58 TYR59 LYS64 ARG68 ILE69 TRP98 SER100 ARG101 GLY102 GLY103 TYR104 ASP105 GLY107 VAL108 SER110 SER111 ASP112 TYR113 SER114 L: ARG33 TYR34 TYR94 ILE96 SER97 THR98 3: PHE57 GLU58 GLY59 ASP60 VAL61 TYR63 VAL64 THR65 THR66 LYS67 THR68 ASP69 SER70 ASP71 ARG72 VAL73 LEU74 PRO114 LYS134 THR135 GLU137 HIS191 LYS193 ASP195 GLY196 ASP197 ALA198 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
