Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3693 |
| PDBID: | 7ECY |
| Chains: | EF_C |
| Organism: | enterovirus D68, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.60 |
| Reference: | 10.1038/s41467-021-23199-5 |
| Antibody | |
| Antibody: | 2H12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Enterovirus D68 (EV-D68) Capsid protein VP2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7ECY_E|Chain D[auth E]|Fab 2H12 heavy chain|Mus musculus (10090) QVQLQQPGAELVMPGASVKMSCKASGYTFTDYWMHWVKQRPGQGLEWIGAIDTSDSYTTYNRKFKGKATLTVDESSSTAYMQLISLTSEDSAVYYCARGGGGNSPFAYWGQGTLVTVSAAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKI |
Light Chain: F
Mutation: NULL
| >7ECY_F|Chain E[auth F]|Fab 2H12 light chain|Mus musculus (10090) DIQMTQSPSSLSASLGERVSLTCRASQDIGSSLNWLQQEPDGTIKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISSLESEDFVDYYCLQYASFPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >7ECY_C|Chain C|Capsid protein VP2|Human enterovirus D68 (42789) SPSAEACGYSDRVLQLKLGNSAIVTQEAANYCCAYGEWPNYLPDHEAVAIDKPTQPETATDRFYTLKSVKWETGSTGWWWKLPDALNNIGMFGQNVQHHYLYRSGFLIHVQCNATKFHQGALLVVAIPEHQRGAHNTNTSPGFDDIMKGEEGGTFNHPYVLDDGTSLACATIFPHQWINLRTNNSATIVLPWMNAAPMDFPLRHNQWTLAIIPVVPLGTRTTSSMVPITVSIAPMCCEFNGLRHAITQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ASP31 TYR32 TRP33 ASP52 SER54 ASP55 TYR57 THR58 THR59 TYR60 ARG62 LYS65 GLY100 GLY101 GLY102 ASN103 SER104 F: SER32 TYR91 ALA92 SER93 PHE94 PRO95 LEU96 C: ARG132 ALA134 HIS135 ASN136 THR137 ASN138 THR139 SER140 PRO141 GLY142 PHE143 ASP144 ASP145 LYS148 PHE155 ASN156 HIS157 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
