Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3692 |
| PDBID: | 7EAN |
| Chains: | HL_A |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.91 |
| Reference: | 10.1038/s41467-021-25997-3 |
| Antibody | |
| Antibody: | 6D6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 Spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7EAN_H|Chain B[auth H]|Heavy chain of SARS-CoV-2 cross-neutralizing mAb 6D6|Mus musculus (10090) EVQLQQSGAELVKPGASVKLSCTTSGFNIIDTYMHWVKQRPEEGLEWIGGIDPVNGNSEYDPKFQDKATITADTSSNTAYLHLSRLTSEDTAVYYCASAHYYGSSSSFPYWGQGTDLVTVSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPWPSETVTCNVAHPASSTKVDKKIVPRG |
Light Chain: L
Mutation: NULL
| >7EAN_L|Chain C[auth L]|Light chain of SARS-CoV-2 cross-neutralizing mAb 6D6|Mus musculus (10090) DIVMTQSQKFMSTSVGDRVSVTCKASQNVGTHVAWYQQKPGQSPKALIYSASYRYSGVPDRFTGSGVGTDFTLTITNVQSEDLAEYFCQQYNSYFTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: A
Mutation: NULL
| >7EAN_A|Chain A|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU1 VAL2 GLY26 PHE27 ASN28 ASP31 THR32 ALA99 HIS100 TYR101 TYR102 GLY103 SER104 TYR110 L: ASN28 GLY30 THR31 HIS32 TYR49 SER50 TYR53 ARG54 TYR55 SER56 VAL67 ASN92 A: TYR351 ALA352 TRP353 ASN354 ARG355 LYS356 ARG357 ASN394 TYR396 PRO426 ASP428 ARG457 SER459 LYS462 PRO463 PHE464 GLU465 ARG466 ILE468 SER469 THR470 GLU471 GLU516 LEU518 ALA520 PRO521 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
