Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3689 |
| PDBID: | 7EAK |
| Chains: | FL_A |
| Organism: | Echovirus E3, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.90 |
| Reference: | 10.1038/s41421-021-00264-3 |
| Antibody | |
| Antibody: | 5G3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Echovirus3 Capsid protein VP1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >7EAK_F|Chain E[auth F]|heavy chain of 5G3 fab|Mus musculus (10090) LQQSGAELARPWASVKISCQAFYTFNSYGMQWVKQRPGQGLEWIGTIYPGNGQTSYNQRFKGKATLTADKSPSTAYMQLISLTSEDSAGCFCAVVPTVDFDYWGQGTLVTVS |
Light Chain: L
Mutation: NULL
| >7EAK_L|Chain F[auth L]|light chain of 5G3 fab|Mus musculus (10090) DIVMSQSPSSLAVSVWEKVTMSCKSSQSLLYSNTQKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYYSYPLTFGAGTKLELKRA |
Antigen
Chain: A
Mutation: NULL
| >7EAK_A|Chain A|Capsid protein VP1|Echovirus E3 (47516) GDVEEAIDRAVARVADTMPTGPRNTESVPALTAVETGHTSQVVPGDTMQTRHVKNYHSRTESSIENFLCRAACVYIATYKSAGGTPTERYASWRINTRQMVQLRRKFELFTYLRFDMEITFVITSTQDPGTQLAQDMPVLTHQIMYIPPGGPVPNSATDFAWQSSTNPSIFWTEGCAPARMSVPFISIGNAYSNFYDGWSHFTQEGVYGFNSLNNMGHIYVRHVNEQSLGVSTSTLRVYFKPKHVRAWVPRPPRLSPYVKSSNVNFKPTAVTTERKDINDVGTLRPVGYTNH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: SER30 TYR51 ASN54 GLN56 THR97 L: GLN27 TYR31 ASN33 TYR38 TYR97 TYR98 TYR100 LEU102 A: THR283 LEU284 ARG285 PRO286 VAL287 GLY288 TYR289 HIS292 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
