Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 367 |
| PDBID: | 2VXT |
| Chains: | HL_I |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.49 |
| Reference: | 10.1074/jbc.M109.023887 |
| Antibody | |
| Antibody: | 125-2H Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-18 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2VXT_H|Chain A[auth H]|MURINE IGG 125-2H|MUS MUSCULUS (10090) EIQLQQSGPELVKPGASVKVSCKASGYSFTDYFIYWVKQSHGKSLEWIGDIDPYNGDTSYNQKFRDKATLTVDQSSTTAFMHLNSLTSEDSAVYFCARGLRFWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCG |
Light Chain: L
Mutation: NULL
| >2VXT_L|Chain C[auth L]|MURINE IGG 125-2H|MUS MUSCULUS (10090) DIQMTQSPSSLSASLGERVSLTCRASQDIGSKLYWLQQEPDGTFKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISSLESEDFVDYYCLQYASSPYTFGGGTKLAIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: I
Mutation: NULL
| >2VXT_I|Chain B[auth I]|INTERLEUKIN-18|HOMO SAPIENS (9606) YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDARDNAPRTIFIISMYKDSQPRGMAVTISVKAEKISTLSAENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLAAEKERDLFKLILKKEDELGDRSIMFTVQNED |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 ASP31 TYR32 PHE33 TYR35 ASP50 TYR53 ASN54 ASP56 THR57 SER58 ARG94 GLY95 LEU96 ARG101 L: ASP28 ILE29 GLY30 SER31 LYS32 TYR34 ARG46 TYR49 ALA50 SER53 ARG66 TYR91 ALA92 SER93 SER94 TYR96 I: LEU51 ASN52 ASP53 PRO93 ARG94 ARG140 VAL142 PRO143 GLY144 HIS145 LYS148 GLU152 GLU157 GLY158 PHE160 LYS175 LYS176 GLU177 ASP178 GLU179 LEU180 GLY181 ASP182 ARG183 MET186 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
