Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3656 |
| PDBID: | 7E72 |
| Chains: | AB_E |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.09 |
| Reference: | 10.1038/s41467-021-26620-1 |
| Antibody | |
| Antibody: | 3H7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Angiopoietin-1 receptor |
| Antigen mutation: | No |
| Durg Target: | Q02763 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7E72_A|Chain A, C|the chimeric Fab fragment of 3H7 (heavy chain)|Homo sapiens (9606) QVQLQQPGAELVRPGASVKLSCKASGYSFTSYWMNWVKQRPGQGLEWIGMIHPSDSETRLNQKFMDKATLTVDKSSSTAYMQLSSPTSEDSAVYYCARGLYGNSWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHHHHHH |
Light Chain: B
Mutation: NULL
| >7E72_B|Chain B, D|the chimeric Fab fragment of 3H7 (light chain)|Homo sapiens (9606) DIQMTQSPSSLSASLGERVSLTCRASQDIGISLNWLQQEPDGTIKRLIYATSSLDSGVPKRFSGSRSGSDYSLTISSLESEDFVDYYCLQYASSPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >7E72_E|Chain E, F|Angiopoietin-1 receptor|Homo sapiens (9606) GSHMIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPES |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER28 THR30 SER31 TYR32 TRP33 HIS52 SER54 ASP55 GLU57 ARG59 LYS74 LEU100 TYR101 ASN103 B: ASP28 ILE29 GLY30 ILE31 SER32 ARG46 TYR49 ALA50 SER53 ARG66 TYR91 ALA92 E: GLU643 ASN644 ILE645 LYS646 ILE647 SER648 ASN649 ILE650 GLN677 GLU705 THR706 ALA707 TYR708 PHE725 SER726 HIS727 GLU728 LEU729 VAL730 THR731 LEU732 PRO733 GLU734 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
