Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3654 |
| PDBID: | 7E6U |
| Chains: | B_A |
| Organism: | Homo sapiens, Camelus dromedarius |
| Method: | EM |
| Resolution (Å): | 6.00 |
| Reference: | 10.7554/eLife.68578 |
| Antibody | |
| Antibody: | NB-2D11 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Extracellular calcium-sensing receptor(CaSR ) |
| Antigen mutation: | No |
| Durg Target: | P41180 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7E6U_B|Chain B, D|NB-2D11|Camelus dromedarius (9838) QVQLQESGGGSVQAGGSLRLSCAASGFPISTYDMGWFRQAPGKEREGVVGITDSFSIKYEDSVKGRFTISRDNAKNALYLQMNSLKPEDTGMYYCAAGDARWSLLLRAEQYNYWGQGTQVTVSSAAAYPYDVPDYGSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7E6U_A|Chain A, C|Extracellular calcium-sensing receptor|Homo sapiens (9606) YGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFLRGHEESGDRFSNSSTAFRPLCTGDENISSVETPYIDYTHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSCIAKEIEFLSWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQIVICVIWLYTAPPSSYRNQELEDEIIFITCHEGSLMALGFLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKIYIILFKPSRNTIEAAADYKDDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN1 VAL2 PHE27 SER30 THR31 TYR32 THR52 ASP53 SER54 SER56 LYS58 ASN73 ALA74 GLY98 ASP99 ALA100 ARG101 TRP102 LEU104 GLN110 ASN112 A: ALA213 ASP215 ARG220 ILE223 GLU224 ARG227 GLU228 ILE237 ASP238 PHE239 SER240 GLU241 LEU242 SER244 TYR246 SER247 ASP248 GLU250 GLU251 HIS254 VAL255 VAL258 GLU556 GLY557 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
