Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3617 |
| PDBID: | 7DNH |
| Chains: | HL_A |
| Organism: | human papillomavirus 58, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.64 |
| Reference: | 10.1128/JVI.01587-20 |
| Antibody | |
| Antibody: | 2H3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human papillomavirus 58 major capsid protein L1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7DNH_H|Chain G[auth H]|The heavy chain of 2H3 Fab fragment|Mus musculus (10090) QVQLLQSGAELVRPGSSVKISCKASGYVFTSYWMHWVKQRPGQGLEWIGQIYPGDGGTHYNGNFRDKATLTADKSSSTAYMHLSSLTSEDSAVYFCARKIYDGYGFSYWGQGTLVTVSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKI |
Light Chain: L
Mutation: NULL
| >7DNH_L|Chain F[auth L]|The light chain of 2H3 Fab fragment|Mus musculus (10090) DIQMTQSPASLSVSVGETVTITCRASENIYSNLIWYQQKQGKSPQLLVYAATNLADGVPSRFSGSGSGTQYSLKINSLQSEDFGSYYCQHFWGTPLTFGAGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >7DNH_A|Chain A, B, C, D, E|Major capsid protein L1|Human papillomavirus type 58 (10598) MVLILCCTLAILFCVADVNVFHIFLQMSVWRPSEATVYLPPVPVSKVVSTDEYVSRTSIYYYAGSSRLLAVGNPYFSIKSPNNNKKVLVPKVSGLQYRVFRVRLPDPNKFGFPDTSFYNPDTQRLVWACVGLEIGRGQPLGVGVSGHPYLNKFDDTETSNRYPAQPGSDNRECLSMDYKQTQLCLIGCKPPTGEHWGKGVACNNNAAATDCPPLELFNSIIEDGDMVDTGFGCMDFGTLQANKSDVPIDICNSTCKYPDYLKMASEPYGDSLFFFLRREQMFVRHFFNRAGKLGEAVPDDLYIKGSGNTAVIQSSAFFPTPSGSIVTSESQLFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMTLCTEVTKEGTYKNDNFKEYVRHVEEYDLQFVFQLCKITLTAEIMTYIHTMDSNILEDWQFGLTPPPSASLQDTYRFVTSQAITCQKTAPPKEKEDPLNKYTFWEVNLKEKFSADLDQFPLGRKFLLQSGLKAKPRLKRSAPTTRAPSTKRKKVKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR30 ASN32 PHE91 TRP92 THR94 H: GLN1 SER25 GLY26 TYR27 VAL28 SER31 TYR32 TRP33 HIS35 GLN50 TYR52 HIS59 LYS99 TYR101 ASP102 GLY103 TYR104 A: ILE52 LYS53 PRO55 ASN56 LEU62 THR346 GLU347 VAL348 THR349 LYS350 GLU351 THR353 LYS355 ASP357 ASN358 PHE359 LYS360 GLU361 TYR362 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
