Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 361 |
| PDBID: | 2VWE |
| Chains: | LJ_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1016/j.jmb.2008.09.076 |
| Antibody | |
| Antibody: | 2H10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vascular endothelial growth factor (VEGF) B |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: L
Mutation: NULL
| >2VWE_L|Chain D[auth E], F[auth L]|ANTI-VEGF-B MONOCLONAL ANTIBODY|MUS MUSCULUS (10090) QVQLQQPGTELVKPGASVKLSCKASGYTFTGFWIHWVKQRPGQGLEWIGHINPGNGGTNYNEKFKRMATLTVDKSSSTAYMQLSSLTSEDSAVYYCARSYSNYVRAMDYWGQGTSVTVSSAKTTAPSVYPLVPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEP |
Light Chain: J
Mutation: NULL
| >2VWE_J|Chain C, E[auth J]|ANTI-VEGF-B MONOCLONAL ANTIBODY|MUS MUSCULUS (10090) EIQMTQTTSSLSASLGDRVTISCRASQDISNFLNWYQQKPDGTVKLLIYYTSTLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGKTLPPTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKEINVKWKIDGSERQNGVLDSWTEQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >2VWE_B|Chain A, B|VASCULAR ENDOTHELIAL GROWTH FACTOR B|HOMO SAPIENS (9606) PVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDSPRPLCPRCTQHHQRPDPRTCRCRCRRRSFLRCQGRGLELNPDTCRCRKLRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: LEU46 TYR49 THR53 LEU54 HIS55 SER56 L: TYR27 THR28 PHE29 THR30 GLY31 PHE32 TRP33 GLY53 ASN54 LYS73 SER76 ARG94 TYR96 SER97 ASN98 ASP101 TYR102 B: SER16 TRP17 ILE18 ASP19 TYR21 THR22 ALA24 THR25 CYS26 GLN27 PRO28 CYS60 PRO62 ASP63 LEU66 GLN100 CYS101 GLU102 CYS103 ARG104 PRO105 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
