Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3598 |
| PDBID: | 7DGE |
| Chains: | D_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.65 |
| Reference: | 10.1007/s13238-020-00808-5 |
| Antibody | |
| Antibody: | Nb43 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Metabotropic glutamate receptor 1 |
| Antigen mutation: | No |
| Durg Target: | Q13255 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7DGE_D|Chain C, D|nanobody|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCAASGRTFTSYAMGWFRQAPGKERESVAAISSSGGSTHYADSVKGRFTISRDNSKNTVYLQMNSLKPEDTAVYYCAAAMYGSRWPDWEYDYWGQGTQVTVSS |
Antigen
Chain: B
Mutation: NULL
| >7DGE_B|Chain A, B|Metabotropic glutamate receptor 1|Homo sapiens (9606) GASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGINRCLPDGQSLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSFDDYFLKLRLDTNHRNPWFQEFWQHRFQCRLEGFPQENSNFKRICTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGSKLLDFLIKSSFIGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWHEGVLNIDDYKIQMNKSGVVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEYVQDEFTCKACDLGWWPNADLTGCEPIPVRYLEWSNIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLN3 ARG29 THR30 THR32 SER33 ALA35 SER54 SER55 SER56 GLY58 SER59 HIS61 MET102 TYR103 GLY104 SER105 ARG106 TRP107 TRP110 GLU111 B: GLN35 ARG36 SER37 VAL38 ARG40 LYS66 GLY150 LYS153 LEU359 ASP360 ASN362 HIS363 ARG364 ASN365 PRO366 GLN369 ARG379 LEU380 GLU381 GLY382 PHE383 PRO384 GLN385 GLU386 SER388 ASN389 ILE393 CYS394 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
