Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3578 |
| PDBID: | 7DC8 |
| Chains: | ED_F |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.76 |
| Reference: | 10.1016/j.celrep.2020.108542 |
| Antibody | |
| Antibody: | Switch-Ab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-6 receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | P08887 |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7DC8_E|Chain B, E|Switch Ab Fab heavy chain|Homo sapiens (9606) EVQLVESGGDLVKPGGGLRLSCAASGFTFSSYTMNWVRQAPGKGLEWVSSISSQSYHIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARYGKLYSLNWVFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: D
Mutation: NULL
| >7DC8_D|Chain A, D|Switch Ab Fab light chain|Homo sapiens (9606) QSALTQPPSASGSPGQTVTISCTGTSTDVGDYAYVSWYQQHPGKAPKLMIYYVSKKPDGVPDRFSGSKSGNTASLTVSGLQAEDEADYFCSLRSPGPYPLFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: F
Mutation: NULL
| >7DC8_F|Chain C, F|Interleukin-6 receptor subunit alpha|Homo sapiens (9606) DVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMSVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSDYKDDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLY28 ASP29 ALA31 TYR32 TYR50 ARG91 SER92 PRO93 GLY94 TYR95 E: HIS56 TYR58 LEU98 TYR99 F: GLU182 GLY183 SER247 PHE248 TYR249 ARG250 ARG252 ASP272 LEU273 GLU296 GLU297 PHE298 GLY299 GLN300 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
