Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3575 |
| PDBID: | 7D8B |
| Chains: | D_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.46 |
| Reference: | 10.1038/s41467-022-32463-1 |
| Antibody | |
| Antibody: | Engineering S4 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Eukaryotic translation initiation factor 4E |
| Antigen mutation: | No |
| Durg Target: | P06730 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7D8B_D|Chain B, D|VH-S4|Homo sapiens (9606) MSEVQLVESGGGLVQPGGSLRLSSAISGFSISSTSIDWVRQAPGKGLEWVARISPSSGSTSYADSVKGRFTISADTSKNTVYLQMNSLRAEDTAVYYTGRVAKALNSRSPSFVVNTYSSIGFDYRGQGTLVTVSSGAAEQKLISEEDLNGAAAFEHHHHHH |
Antigen
Chain: C
Mutation: NULL
| >7D8B_C|Chain A, C|Eukaryotic translation initiation factor 4E|Homo sapiens (9606) MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: VAL3 GLN4 LEU5 ALA101 LYS102 ALA103 LEU104 ARG107 PHE111 THR115 TYR116 SER118 ILE119 PHE121 ASP122 TYR123 ARG124 GLY125 GLN126 C: HIS33 TYR34 ILE35 HIS37 LEU39 VAL69 GLU70 TRP73 TYR76 ASN77 ARG128 LEU131 GLU132 LEU135 ILE138 GLY139 ARG186 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
