Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3572 |
| PDBID: | 7D85 |
| Chains: | BC_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1158/1535-7163.MCT-20-0907 |
| Antibody | |
| Antibody: | ISU104 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Barecetamab(Phase-I) |
| Antigen | |
| Antigen: | Receptor tyrosine-protein kinase erbB-3 |
| Antigen mutation: | No |
| Durg Target: | P21860 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7D85_B|Chain B, E|Anti-ErbB3 Fab heavy chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSDYDMSWVRQAPGKGLEWVSTIDLDSGSIYYADSVQGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDLHMGPEGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: C
Mutation: NULL
| >7D85_C|Chain C, F|Anti-ErbB3 Fab light chain|Homo sapiens (9606) QSVLTQPPSASGTPGQRVTISCSGSSSNIGSNSVSWYQQLPGTAPKLLIYSDNHRPSGVPDRFSGSKSGTSASLAISGLRSEDEADYYCQGWDTSLSGHVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >7D85_A|Chain A, D|Receptor tyrosine-protein kinase erbB-3|Homo sapiens (9606) GIPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP33 THR50 ASP52 LEU53 ASP54 GLY56 SER57 TYR59 ASP99 LEU100 HIS101 MET102 GLY103 PRO104 PRO107 C: GLY30 SER31 ASN32 SER33 TYR50 SER51 ASP52 ASN53 HIS54 SER57 LYS67 TRP92 THR94 SER97 HIS99 A: GLY316 SER317 GLY318 SER319 ARG320 GLN322 LEU345 TRP354 GLN381 GLY401 ARG402 SER403 LEU404 TYR405 ASN406 ARG407 GLY408 PHE409 LEU412 MET414 LYS415 GLY433 ARG434 TYR436 GLU461 ASP464 LYS466 HIS467 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
