Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 356 |
| PDBID: | 2VH5 |
| Chains: | HL_R |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.jmb.2007.11.085 |
| Antibody | |
| Antibody: | ANTI-RAS Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HRAS(G12V) |
| Antigen mutation: | No |
| Durg Target: | P01112; P01116; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2VH5_H|Chain A[auth H]|ANTI-RAS FV HEAVY CHAIN|HOMO SAPIENS (9606) EVQLLESGGGLVQPGGSLRLSAAASGFTFSTFSMNWVRQAPGKGLEWVSYISRTSKTIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYVARGRFFDYWGQGTLVTVS |
Light Chain: L
Mutation: NULL
| >2VH5_L|Chain B[auth L]|ANTI-RAS FV LIGHT CHAIN|HOMO SAPIENS (9606) IQMTQSPSSLSASVGDRVTITVRASQSISSYLNWYQQKPGEAPKLLIYSASVLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYAQQSVMIPMTFGQGTKVE |
Antigen
Chain: R
Mutation: NULL
| >2VH5_R|Chain C[auth R]|GTPASE HRAS|HOMO SAPIENS (9606) MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 SER30 THR31 PHE32 SER33 TYR50 SER52 ARG53 THR54 LYS56 THR57 TYR59 ARG98 GLY99 ARG100 PHE101 L: SER32 TYR34 VAL94 MET95 ILE96 MET98 R: VAL12 SER17 ILE21 GLN25 HIS27 VAL29 GLU31 TYR32 ASP33 PRO34 THR35 ILE36 GLU37 ASP38 SER39 TYR40 LEU56 ASP57 GLN61 GLU63 TYR64 MET67 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
