Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3525 |
| PDBID: | 7CZ0 |
| Chains: | G_C |
| Organism: | Galaxea fascicularis, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.77 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | 92 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Thermostable green fluorescent protein (TGP) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: G
Mutation: NULL
| >7CZ0_G|Chain E, F, G, H|Synthetic nanobody (Sybody) 92 recognizing the thermostable green fluorescent protein (TGP)|synthetic construct (32630) GSSSQVQLVESGGGLVQAGGSLRLSCAASGFPVDTQWMHWYRQAPGKEREWVAAISSTGRSTFYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCTVYVGNRYRGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
Antigen
Chain: C
Mutation: NULL
| >7CZ0_C|Chain A, B, C, D|Thermostable green fluorescent protein (TGP)|Galaxea fascicularis (46745) MASVIKPEMKIKLRMEGAVNGHKFVIEGEGIGKPYEGTQTLDLTVEEGAPLPFSYDILTPAFQYGNRAFTKYPEDIPDYFKQAFPEGYSWERSMTYEDQGICIATSDITMEGDCFFYEIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVEMALLLEGGGHYRCDFKTTYKAKKDVRLPDAHEVDHRIEILSHDKDYNKVRLYEHAEARYSGGGSGGGHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: TRP33 HIS35 TYR37 ARG45 TRP47 ALA50 SER52 SER53 THR54 ARG56 SER57 THR58 PHE59 TYR60 ALA61 ASP62 LYS65 THR97 VAL98 TYR99 GLY101 ARG103 C: LYS12 LEU13 ARG14 GLU16 GLU27 GLU29 PRO85 GLU86 ASP107 THR109 GLU111 GLY112 PHE116 TYR117 GLU118 LYS181 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
