Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3475 |
| PDBID: | 7COE |
| Chains: | HL_A |
| Organism: | Homo sapiens, Middle East respiratory syndrome-related coronavirus |
| Method: | XRD |
| Resolution (Å): | 2.05 |
| Reference: | 10.1038/s41598-022-05318-4 |
| Antibody | |
| Antibody: | KNIH90-F1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MERS-CoV Spike glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7COE_H|Chain A[auth B], D[auth H]|Heavy chain|Homo sapiens (9606) QVQLVQSGAEVKRPGSSVKVSCKTSGGTFNNNAINWVRQAPGQGLEWMGGIIPFFGIAKYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARDLPRESSYGSGSYYTHYYAMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKT |
Light Chain: L
Mutation: NULL
| >7COE_L|Chain B[auth C], E[auth L]|Light chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCGASQSVSSSYLAWYQQKPGLAPRLLIYDASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7COE_A|Chain C[auth A], F[auth D]|Spike glycoprotein|Middle East respiratory syndrome-related coronavirus (1335626) EAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN31 ILE52 PRO53 PHE54 PHE55 GLY56 ILE57 ALA58 LYS59 GLU74 SER105 TYR106 SER110 TYR111 TYR112 THR113 HIS114 TYR115 L: TYR92 GLY93 SER94 SER95 LEU97 A: GLN466 PHE467 ASN501 LYS502 SER504 ARG505 LEU506 ASP509 ASP510 ARG511 THR512 GLU513 VAL514 PRO515 LEU517 TYR540 ARG542 TRP553 VAL555 SER557 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
