Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3469 |
| PDBID: | 7CN2 |
| Chains: | HL_D |
| Organism: | Oryctolagus cuniculus, Human papillomavirus 16 |
| Method: | EM |
| Resolution (Å): | 3.43 |
| Reference: | 10.1038/s41541-020-00236-w |
| Antibody | |
| Antibody: | H16.001 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | papillomavirus type 16 pesudovirus major capsid protein L1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7CN2_H|Chain B[auth G], D[auth H], F[auth I], H[auth J], J[auth K], L[auth M]|The heavy chain variable region of H16.001 Fab fragment|Oryctolagus cuniculus (9986) QSVKESEGRLVTPGTPLTLTCTASGFTMSRYHMTWVRQAPGKGLEWIGIIYARNSDTYYANWAKGRFTISKTSTTVDLKITSPTIEDTATYFCARVDSDSSGAFDRLDLWGQGTLVTVSS |
Light Chain: L
Mutation: NULL
| >7CN2_L|Chain A[auth L], C[auth g], E[auth i], G[auth j], I[auth k], K[auth m]|The light chain variable region of H16.001 Fab fragment|Oryctolagus cuniculus (9986) DPMLTQTAASVEVAVGGTVTIKCQASQSIGGYLSWYQQKPGQRPKLLIYRASTLASGVPSRFKGSGSGTEYTLTFSGVECADAAAYYCQQGYTSSDINNAFGGGTEVVVK |
Antigen
Chain: D
Mutation: NULL
| >7CN2_D|Chain M[auth A], N[auth B], O[auth C], P[auth D], Q[auth E], R[auth F]|Major capsid protein L1|Human papillomavirus type 16 (333760) MSLWLPSEATVYLPPVPVSKVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIHLPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASAYAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTNVAVNPGDCPPLELINTVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYLRREQMFVRHLFNRAGAVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQAIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQAGLKAKPKFTLGKRKATPTTSSTSTTAKRKKRKL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 TYR49 ARG50 SER67 THR93 H: SER29 ARG30 TYR31 HIS32 TYR51 ALA52 ARG53 ASN54 TYR58 ASP97 SER98 ASP99 SER100 SER101 GLY102 ALA103 ARG106 D: LEU126 ASP127 TYR135 ALA136 ALA137 ASN138 ALA139 GLY140 VAL141 ASP142 ASN143 GLU145 ASN181 ALA264 GLY265 ALA266 VAL267 GLY281 SER282 ASN285 LEU286 ALA287 SER288 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
