Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3448 |
| PDBID: | 7CBP |
| Chains: | RS_N |
| Organism: | Zika virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.10 |
| Reference: | 10.1016/j.yjsbx.2020.100028 |
| Antibody | |
| Antibody: | ZAb_FLEP Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Zika virus envelope protein E |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: R
Mutation: NULL
| >7CBP_R|Chain G[auth D], I[auth R]|Fab Heavy chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFSFSTYSMHWVRQAPGKGLEWVSAISGEGDSAYYADSVKGRFTISRDNSKNTLYLQMNKVRAEDTAVYYCVGGYSNFYYYYTMDAWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: S
Mutation: NULL
| >7CBP_S|Chain H[auth E], J[auth S]|Fab light chain|Homo sapiens (9606) DIVMTQSPASLSLSPGERATLSCRATQSISTFLAWYQQKPGQAPRLLIYDASTRASGIPARFSGSRSGTDFTLTITRLEPEDFAVYYCQQRYNWPPYSFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: N
Mutation: NULL
| >7CBP_N|Chain D[auth K], E[auth N], F[auth Q]|Envelope protein E|Zika virus (64320) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition R: SER30 TYR100 SER101 ASN102 TYR106 THR108 MET109 S: SER30 THR31 PHE32 TYR49 ASP50 SER52 THR53 ARG54 ALA60 SER63 GLY64 SER65 ARG66 N: ARG2 HIS148 SER149 GLY150 MET151 ASN154 ASP155 THR156 HIS158 GLU159 ARG283 THR315 LYS316 ILE317 GLN331 THR369 GLU370 ASN371 LYS373 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
