Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3447 |
| PDBID: | 7CBP |
| Chains: | DE_K |
| Organism: | Zika virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.10 |
| Reference: | 10.1016/j.yjsbx.2020.100028 |
| Antibody | |
| Antibody: | ZAb_FLEP Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Zika virus envelope protein E |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7CBP_D|Chain G[auth D], I[auth R]|Fab Heavy chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFSFSTYSMHWVRQAPGKGLEWVSAISGEGDSAYYADSVKGRFTISRDNSKNTLYLQMNKVRAEDTAVYYCVGGYSNFYYYYTMDAWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: E
Mutation: NULL
| >7CBP_E|Chain H[auth E], J[auth S]|Fab light chain|Homo sapiens (9606) DIVMTQSPASLSLSPGERATLSCRATQSISTFLAWYQQKPGQAPRLLIYDASTRASGIPARFSGSRSGTDFTLTITRLEPEDFAVYYCQQRYNWPPYSFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: K
Mutation: NULL
| >7CBP_K|Chain D[auth K], E[auth N], F[auth Q]|Envelope protein E|Zika virus (64320) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLU54 GLY55 ASP56 SER57 ALA58 TYR59 TYR60 ASP62 LYS65 GLY66 THR69 ASN102 PHE103 TYR104 TYR105 TYR106 E: GLN27 SER28 SER30 THR31 PHE32 ARG91 TYR92 ASN93 TRP94 K: ASP67 MET68 ALA69 SER70 ASP71 SER72 ARG73 CYS74 GLN77 ASP83 LYS84 VAL97 ASP98 ARG99 TRP101 GLY102 ASN103 GLY104 CYS105 GLY106 LEU113 LYS251 ARG252 GLN253 VAL255 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
