Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3433 |
| PDBID: | 7C6A |
| Chains: | HL_A |
| Organism: | Mus musculus, Homo sapiens, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/s41598-020-68355-x |
| Antibody | |
| Antibody: | SRP2070 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Soluble cytochrome b562 |
| Antigen mutation: | Yes |
| Durg Target: | P50052; P50052; P0ABE7; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7C6A_H|Chain B[auth H]|IgG heavy chain|Mus musculus (10090) QIQLQQSGPELVKPGASVKISCKASGYTFTDFYINWMKQRPGQGLEWIGWIFPGSGNTHYNEKFKGKATLIVDTSSSTAFMQLNSLTSEDSAVYFCTRPVSYYYDFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIK |
Light Chain: L
Mutation: NULL
| >7C6A_L|Chain A[auth L]|IgG Light Chain|Mus musculus (10090) DIVLTQSPATLSVTPGDRVSLSCRASQSVSNYLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPLTFGAGTKLELRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: L60V/F100W/M216W/H311I/R315L
| >7C6A_A|Chain C[auth A]|Type-2 angiotensin II receptor,Soluble cytochrome b562,Type-2 angiotensin II receptor|Homo sapiens (9606) GCSQKPSDKHLDAIPILYYIIFVIGFLVNIVVVTLFCCQKGPKKVSSIYIFNLAVADLLVLATLPLWATYYSYRYDWLFGPVMCKVFGSFLTLNMFASIWFITCMSVDRYQSVIYPFLSQRRNPWQASYIVPLVWCMACLSSLPTFYFRDVRTIEYLGVNACIMAFPPEKYAQWSAGIALMKNILGFIIPLIFIATCYFGIRKHLLKTNADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLKNRITRDQVLKMAAAVVLAFIICWLPFHVLTFLDALAWMGVINSCEVIAVIDLALPFAILLGFTNSCVNPFLYCFVGNRFQQKLRSVFRVPITWLQGKRESENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition L: GLN27 TYR32 TYR50 SER91 ASN92 SER93 TRP94 LEU96 H: THR28 ASP31 PHE32 TYR33 ASN35 TRP50 PHE52 GLY54 SER55 ASN57 THR58 HIS59 PRO99 VAL100 SER101 TYR102 TYR103 TYR104 A: GLU1018 HIS1063 ILE1067 GLY1070 GLN1071 ASP1073 ASP1074 LYS1077 LEU1078 LYS1083 LYS1085 GLU1086 GLN1088 ALA1089 ALA1090 GLU1092 GLN1093 LYS1095 THR1096 THR1097 ARG1098 ASN1099 ALA1100 TYR1101 GLN1103 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
