Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3432 |
| PDBID: | 7C61 |
| Chains: | HL_A |
| Organism: | Mus musculus, Homo sapiens, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/s41598-020-68355-x |
| Antibody | |
| Antibody: | SRP2070 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | thermostabilised apocytochrome b562 from Escherichia coli M7W/H102I/R106L (BRIL) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7C61_H|Chain B[auth H]|IGG HEAVY CHAIN|Mus musculus (10090) QIQLQQSGPELVKPGASVKISCKASGYTFTDFYINWMKQRPGQGLEWIGWIFPGSGNTHYNEKFKGKATLIVDTSSSTAFMQLNSLTSEDSAVYFCTRPVSYYYDFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKC |
Light Chain: L
Mutation: NULL
| >7C61_L|Chain A[auth L]|IGG LIGHT CHAIN|Mus musculus (10090) DIVLTQSPATLSVTPGDRVSLSCRASQSVSNYLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPLTFGAGTKLELRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: L107W/M215W/H310I/R314L
| >7C61_A|Chain C[auth A]|5-hydroxytryptamine receptor 1B,Soluble cytochrome b562,5-hydroxytryptamine receptor 1B|Homo sapiens (9606) GCSAKDYIYQDSISLPWKVLLVMLLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASIWHLCVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFFWRQAKAEEEVSECVVNTDHILYTVYSTVGAFYFPTLLLIALYGRIYVEARSRIADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAIFDFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKCTS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 TYR50 SER91 ASN92 SER93 TRP94 LEU96 H: THR28 THR30 ASP31 PHE32 TYR33 ASN35 TRP50 PHE52 GLY54 SER55 ASN57 THR58 HIS59 GLU62 PRO99 VAL100 SER101 TYR102 TYR103 TYR104 A: GLU257 ASP299 HIS302 ILE306 GLY309 GLN310 ASP312 ASP313 LYS316 LEU317 GLU320 LYS322 LYS324 GLU325 GLN327 ALA328 ALA329 GLU331 GLN332 LYS334 THR335 THR336 ARG337 ASN338 ALA339 TYR340 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
