Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 342 |
| PDBID: | 2R4R |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/nature06325 |
| Antibody | |
| Antibody: | anti-beta2 adrenoceptor Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-2 adrenergic receptor |
| Antigen mutation: | No |
| Durg Target: | P07550 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2R4R_H|Chain C[auth H]|antibody for beta2 adrenoceptor, heavy chain|Mus musculus (10090) EVQLQQSGAELARPGASVKLSCKASGYIFTDYYINWVRQRTGQGFEWIGEIYPGSGNIDYNERFKDKATLTADKSSSTAYMQLSSLTSEDSAVYFCVRGFGYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSAVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGC |
Light Chain: L
Mutation: NULL
| >2R4R_L|Chain B[auth L]|antibody for beta2 adrenoceptor, light chain|Mus musculus (10090) DIKMTQSPSSMYASLGERVTITCKASQDINSYLSWFQQKPGKSPKTLIYRANRLVDGVPSRFIGTGSGQDYSLTISSLDYADMGIYYCLQYDEFPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >2R4R_A|Chain A|Beta-2 adrenergic receptor|Homo sapiens (9606) MGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 SER34 THR46 TYR49 ARG50 VAL55 ASP56 TYR91 ASP92 GLU93 PHE94 TYR96 H: ASP31 TYR32 TYR33 GLU50 TYR52 GLY54 SER55 GLY56 ASN57 SER75 ARG98 GLY99 PHE100 GLY101 A: LYS227 LEU230 GLN231 ILE233 ASP234 LYS235 SER236 GLU237 GLY238 ARG239 PHE240 HIS241 VAL242 CYS265 LEU266 LYS267 HIS269 LYS270 LEU347 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
