Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 340 |
| PDBID: | 2R0L |
| Chains: | HL_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1073/pnas.0708251104 |
| Antibody | |
| Antibody: | 75 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | hepatocyte growth factor activator(HGFA) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2R0L_H|Chain B[auth H]|antibody heavy chain, Fab portion only|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTISNSGIHWVRQAPGKGLEWVGWIYPTGGATDYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARFWWRSFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >2R0L_L|Chain A[auth L]|antibody light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >2R0L_A|Chain C[auth A]|Hepatocyte growth factor activator|Homo sapiens (9606) IIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGVYTRVANYVDWINDRIRPPRRLVAPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASP28 SER30 SER91 TYR92 THR93 THR94 PRO96 H: ASN31 SER32 HIS35 TRP47 TRP50 PRO52 THR53 GLY54 GLY55 ALA56 THR57 ASP58 ARG94 PHE95 TRP96 TRP97 ARG98 A: PHE59 PRO60 ARG61 LYS87 TYR88 ILE89 PRO90 TYR91 THR92 LEU93 TYR94 SER95 VAL96 PHE97 ASN98 ASP100 HIS101 SER177 PRO178 ASN179 ASN233 TRP237 ARG241 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
