Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 34 |
| PDBID: | 1FE8 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.03 |
| Reference: | 10.1074/jbc.M006548200 |
| Antibody | |
| Antibody: | RU5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | von Willebrand factor |
| Antigen mutation: | No |
| Durg Target: | P04275 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >1FE8_H|Chain B[auth H], E[auth I], H[auth J]|IMMUNOGLOBULIN IGG RU5|Mus musculus (10090) DVKLVQSGPGLVAPSQSLSITCTVSGFSLTTYGVSWVRQPPGKGLEWLGVIWGDGNTTYHSALISRLSISKDNSRSQVFLKLNSLHTDDTATYYCAGNYYGMDYWGQGTSVTVSSAETTAPSVYKLEPVSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRG |
Light Chain: L
Mutation: NULL
| >1FE8_L|Chain C[auth L], F[auth M], I[auth N]|IMMUNOGLOBULIN IGG RU5|Mus musculus (10090) DIAMTQTTSSLSASLGQKVTISCRASQDIGNYLNWYQQKPDGTVRLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLESEDIATYFCQNGGTNPWTFGGGTKLEVKRADAAPTTSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: A
Mutation: NULL
| >1FE8_A|Chain A, D[auth B], G[auth C]|VON WILLEBRAND FACTOR|Homo sapiens (9606) GSHMAPDCSQPLDVILLLDGSSSFPASYFDEMKSFAKAFISKANIGPRLTQVSVLQYGSITTIDVPWNVVPEKAHLLSLVDVMQREGGPSQIGDALGFAVRYLTSEMHGARPGASKAVVILVTDVSVDSVDAAADAARSNRVTVFPIGIGDRYDAAQLRILAGPAGDSNVVKLQRIEDLPTMVTLGNSFLHKLCSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 THR31 TRP52 GLY53 ASP54 ASN56 ASN98 TYR99 TYR100 GLY101 L: ASP28 ILE29 GLY30 ASN31 TYR32 TYR49 TYR50 ARG53 SER67 GLY68 GLY91 GLY92 THR93 ASN94 TRP96 A: GLY961 PRO962 ARG963 GLN966 ASP979 PRO981 TRP982 ASN983 VAL984 VAL985 PRO986 GLU987 ALA989 HIS990 LEU992 SER993 LEU994 ASP996 VAL997 GLU1021 HIS1023 GLY1024 ARG1026 PRO1027 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
