Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 339 |
| PDBID: | 2R0K |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.51 |
| Reference: | 10.1073/pnas.0708251104 |
| Antibody | |
| Antibody: | 58 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | hepatocyte growth factor activator(HGFA) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2R0K_H|Chain C[auth H]|antibody heavy chain of Fab58, Fab portion only|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTITGSAIHWVRQAPGKGLEWVAIINPNGGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSARFSFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >2R0K_L|Chain B[auth L]|antibody light chain of Fab58|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >2R0K_A|Chain A|Hepatocyte growth factor activator|Homo sapiens (9606) VQLSPDLLATLPEPASPGRQACGRRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSVFNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGVYTRVANYVDWINDRIRPPRRLVAPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER91 TYR92 THR93 THR94 PRO96 H: PHE27 THR28 THR30 GLY31 SER32 ALA33 HIS35 ILE50 ASN53 GLY54 TYR56 THR73 SER74 ASN76 ARG94 SER95 ALA96 ARG97 PHE98 PHE100 A: HIS57 SER95 VAL96 PHE97 ASN98 SER99 GLU146 ASN147 TYR172 ALA174 ASP175 MET180 GLN192 SER214 TRP215 GLY216 ASP217 GLY219 ARG221 LYS224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
