Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3389 |
| PDBID: | 7BC6 |
| Chains: | B_A |
| Organism: | Gallus gallus, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/s41586-021-03579-z |
| Antibody | |
| Antibody: | 7BC6 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | proton-coupled folate transporter (PCFT) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7BC6_B|Chain B|nanobody|Lama glama (9844) GPSQVQLVESGGGLVQPGGSLRLSCAASGFTFSRYWMYWVRQAPGKGPEWLSHMNPSGSDIKYTDSVKGRFTISRDNAKNTLYLQMNSLKPDDTAVYYCVADRRALGSPEYWGQGTQVTVSSA |
Antigen
Chain: A
Mutation: NULL
| >7BC6_A|Chain A|Proton-coupled folate transporter|Gallus gallus (9031) MAAPSDPPTAATPPAPPPPARRCLPAPSVEPLLFLATLALGLQVPLATQYLWDRLGAERGYVGPNASSPHGCGNGSGAVDPLREEVEALVAHWNLCINLGGFFVGLFSVTLFGPWSDSVGRRPVLVLPAVGMAVQAAVYLLVMYLRLHVAYLLLGRIISGLLGDYNLILAGCFASVADSSNQRTRTFRVAILEACLGVAGMVASVGGGQWRKAEGYINPFWLVLAASLAAALYAALCLQETVKQRRAAKLLTLQHYKAVYKLYTAPEDLSSRRKLALYSLAFFLLVTVHFGTKDLYVLYELGSPLCWASDLIGYGSAASYLAYLSSLGGLRLLQLCLEDTWVAEIGLISNIAGLVVISLATTTPLMFTGYGIMFLSMAATPVIRAKLSKLVSETEQGALFASVACVEGLCSLVATGVFNSLYPSTLHFMRGFPFLFGAILLLIPAAIMGWIEIQDSNLQYSHFSDASSSPADGGENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: TYR32 TRP33 TYR35 GLN39 LYS43 GLY44 PRO45 TRP47 SER49 HIS50 LYS59 TYR60 THR61 TYR95 VAL97 ASP99 ARG100 ARG101 ALA102 LEU103 GLY104 SER105 PRO106 GLU107 TYR108 TRP109 GLY110 A: PRO45 THR48 GLN49 TRP52 ASP53 ALA57 GLY60 TYR61 VAL62 GLY63 PRO64 ASN65 ALA66 SER67 PRO69 GLY73 ASN74 GLY75 SER76 ALA78 VAL79 ASP80 PRO81 ARG83 ARG211 LYS212 ALA213 TYR216 VAL297 LEU301 TRP307 ALA308 SER309 ILE312 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
