Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3387 |
| PDBID: | 7BBJ |
| Chains: | HL_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.72 |
| Reference: | 10.1158/1535-7163.MCT-21-0107 |
| Antibody | |
| Antibody: | mAb19 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | CD73 protein |
| Antigen mutation: | Yes |
| Durg Target: | P21589 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7BBJ_H|Chain C[auth H], E[auth M]|heavy chain mAb19| synthetic construct (32630) QVQLVQSGAEVKKPGDSVKVSCKASGYTFTTYWMHWVRQAPGQGLEWMGAIYPGLSDTTYNQKFKGKVTMTRDTSTSTVYMKLSSLRSEDTAVYYCARLLDYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: L
Mutation: NULL
| >7BBJ_L|Chain D[auth L], F[auth N]|light chain mAB19| synthetic construct (32630) DIQMTQSPSSLSASVGDRVTITCRASQDIRSYLNWHQQKPGKAPKLLIWYTSRLHSGVPSRFSGSGSGTDYTLTISSLQPEDFATYFCQQGETLPWTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: N50D/K142S/K144S/N308D/N330D/N400D/K475S
| >7BBJ_A|Chain A, B|5'-nucleotidase|Homo sapiens (9606) MAHHHHHHVGTGSNDDDDKSPDPWELTILHTNDVHSRLEQTSEDSSKCVDASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANISASGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLDSSIPEDPSIKADINKWRIKLDDYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNDGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTSCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 THR31 TYR32 TRP33 TYR52 LEU55 ASP57 LEU99 LEU100 ASP101 TYR102 L: ARG30 SER31 TYR32 TRP49 TYR50 THR51 SER52 ARG53 SER65 GLY66 SER67 GLY91 GLU92 A: LEU132 PHE137 PRO138 ILE139 ALA151 SER152 GLN153 SER155 GLY156 LEU157 TYR158 LEU159 PRO160 TYR161 LYS162 VAL163 PRO165 GLY167 ASP168 GLU169 VAL170 GLU203 LYS206 THR209 LEU210 ASN211 VAL212 ASN213 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
