Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3385 |
| PDBID: | 7B5G |
| Chains: | H_G |
| Organism: | Escherichia coli K-12, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1016/j.str.2022.09.004 |
| Antibody | |
| Antibody: | SOS3 (14527) Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Escherichia coli K-12 lexA repressor |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >7B5G_H|Chain B, D, F, H|Nanobody Nb14527, NbSOS3|Lama glama (9844) QVQLVESGGGVVQAGGSLRLSCAASGSIFSSNAMAWYRQAPGNVRRLVAAISSRGDNTNYEDSVRGRFTISRDNAENTVSLQMNSLKPEDTAIYYCNVGSFYRGNYYGGSSWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: G
Mutation: NULL
| >7B5G_G|Chain A, C, E, G|LexA repressor|Escherichia coli K-12 (83333) MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR37 GLN39 GLY42 ASN43 VAL44 ARG45 ARG46 LEU47 GLU61 GLY99 SER100 PHE101 TYR102 ARG103 ASN105 TYR106 TYR107 GLY108 GLY109 SER110 TRP112 G: GLU74 GLY75 LEU76 PRO77 GLY85 PRO87 LEU88 LEU89 ALA90 GLN91 ILE94 HIS97 ASP110 PHE111 LYS135 GLN137 ASP138 ARG148 ASP150 ASP151 LYS160 GLY162 ASN163 VAL165 LEU181 ARG182 GLN184 SER185 PHE186 THR187 ILE188 GLU189 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
