Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3380 |
| PDBID: | 7B2Q |
| Chains: | D_C |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.76 |
| Reference: | 10.4049/jimmunol.2100647 |
| Antibody | |
| Antibody: | Anti-C4b B12 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | complement C4b gamma chain |
| Antigen mutation: | No |
| Durg Target: | P0C0L5; P0C0L4; |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7B2Q_D|Chain D|Anti-C4b nanobody B12|Lama glama (9844) EVQLVESGGGLVQAGGSLRLSCVASERTYMAWFRQAPGKEREFVAAITSSGMMTEYAPSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAADLRQRFGERVTEYDYWGQGTQVTVSSHGSGLVPRGSGGGHHHHHH |
Antigen
Chain: C
Mutation: NULL
| >7B2Q_C|Chain C|Complement C4 gamma chain|Homo sapiens (9606) EAPKVVEEQESRVHYTVCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLLYFDSVPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDSNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLU26 ARG27 THR28 THR48 SER49 SER50 MET52 MET53 ASP95 LEU96 ARG97 GLN98 ARG99 PHE100 C: ARG1465 VAL1466 HIS1467 THR1469 LEU1497 ASP1500 LYS1503 LEU1504 LEU1507 SER1508 ASP1509 TYR1511 ARG1533 GLU1534 CYS1535 VAL1536 GLY1537 PHE1538 GLU1539 VAL1541 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
