Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3379 |
| PDBID: | 7B2Q |
| Chains: | D_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.76 |
| Reference: | 10.4049/jimmunol.2100647 |
| Antibody | |
| Antibody: | Anti-C4b B12 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | complement C4b alpha chain |
| Antigen mutation: | Yes |
| Durg Target: | P0C0L4; P0C0L5; |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7B2Q_D|Chain D|Anti-C4b nanobody B12|Lama glama (9844) EVQLVESGGGLVQAGGSLRLSCVASERTYMAWFRQAPGKEREFVAAITSSGMMTEYAPSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAADLRQRFGERVTEYDYWGQGTQVTVSSHGSGLVPRGSGGGHHHHHH |
Antigen
Chain: B
Mutation: T522S
| >7B2Q_B|Chain B|Complement C4 alpha chain|Homo sapiens (9606) NVNFQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALSLTKAPVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: MET53 GLU55 PHE100 GLY101 B: LYS1404 GLY1405 HIS1406 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
