Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3378 |
| PDBID: | 7B2P |
| Chains: | D_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.43 |
| Reference: | 10.4049/jimmunol.2100647 |
| Antibody | |
| Antibody: | Anti-C4b B5 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | complement C4b beta chain |
| Antigen mutation: | No |
| Durg Target: | P0C0L4; P0C0L5; |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7B2P_D|Chain D|Nanobody B5|Lama glama (9844) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYHMSWVRQAPGKGLEWISVINDSGDLTRYADSVKGRFTISRDNAKNTLYLQMNSLQPEDTAVYSCLKSSDFYSYSNADSRGQGTQVTVSSHGSGLVPRGSGGGHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7B2P_A|Chain A|Complement C4 beta chain|Homo sapiens (9606) KPRLLLFSPSVVHLGVPLSVGVQLQDVPRGQVVKGSVFLRNPSRNNVPCSPKVDFTLSSERDFALLSLQVPLKDAKSCGLHQLLRGPEVQLVAHSPWLKDSLSRTTNIQGINLLFSSRRGHLFLQTDQPIYNPGQRVRYRVFALDQKMRPSTDTITVMVENSHGLRVRKKEVYMPSSIFQDDFVIPDISEPGTWKISARFSDGLESNSSTQFEVKKYVLPNFEVKITPGKPYILTVPGHLDEMQLDIQARYIYGKPVQGVAYVRFGLLDEDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESPGGEMEEAELTSWYFVSSPFSLDLSKTKRHLVPGAPFLLQALVREMSGSPASGIPVKVSATVSSPGSVPEVQDIQQNTDGSGQVSIPIIIPQTISELQLSVSAGSPHPAIARLTVAAPPSGGPGFLSIERPDSRPPRVGDTLNLNLRAVGSGATFSHYYYMILSRGQIVFMNREPKRTLTSVSVFVDHHLAPSFYFVAFYYHGDHPVANSLRVDVQAGACEGKLELSVDGAKQYRNGESVKLHLETDSLALVALGALDTALYAAGSKSHKPLNMGKVFEAMNSYDLGCGPGGGDSALQVFQAAGLAFSDGDQWTLSRKRLSCPKEKTT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: GLU46 TRP47 ASP53 ARG59 ASP62 ASP101 ASN107 A: ARG157 GLY584 SER586 LYS588 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
