Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3377 |
| PDBID: | 7B2P |
| Chains: | D_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.43 |
| Reference: | 10.4049/jimmunol.2100647 |
| Antibody | |
| Antibody: | Anti-C4b B5 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | complement C4b alpha chain |
| Antigen mutation: | Yes |
| Durg Target: | P0C0L4; P0C0L5; |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7B2P_D|Chain D|Nanobody B5|Lama glama (9844) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYHMSWVRQAPGKGLEWISVINDSGDLTRYADSVKGRFTISRDNAKNTLYLQMNSLQPEDTAVYSCLKSSDFYSYSNADSRGQGTQVTVSSHGSGLVPRGSGGGHHHHHH |
Antigen
Chain: B
Mutation: T522S
| >7B2P_B|Chain B|Complement C4 alpha chain|Homo sapiens (9606) NVNFQKAINEKLGQYASPTAKRCCQDGVTRLPMMRSCEQRAARVQQPDCREPFLSCCQFAESLRKKSRDKGQAGLQRALEILQEEDLIDEDDIPVRSFFPENWLWRVETVDRFQILTLWLPDSLTTWEIHGLSLSKTKGLCVATPVQLRVFREFHLHLRLPMSVRRFEQLELRPVLYNYLDKNLTVSVHVSPVEGLCLAGGGGLAQQVLVPAGSARPVAFSVVPTAAAAVSLKVVARGSFEFPVGDAVSKVLQIEKEGAIHREELVYELNPLDHRGRTLEIPGNSDPNMIPDGDFNSYVRVTASDPLDTLGSEGALSPGGVASLLRLPRGCGEQTMIYLAPTLAASRYLDKTEQWSTLPPETKDHAVDLIQKGYMRIQQFRKADGSYAAWLSRDSSTWLTAFVLKVLSLAQEQVGGSPEKLQETSNWLLSQQQADGSFQDPCPVLDRSMQGGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISKANSFLGEKASAGLLGAHAAAITAYALSLTKAPVDLLGVAHNNLMAMAQETGDNLYWGSVTGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQGSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: LEU45 TRP47 ILE48 SER49 VAL50 LEU57 THR58 ARG59 TYR60 ALA61 ASP62 LYS65 SER99 SER100 ASP101 PHE102 TYR103 SER104 TYR105 SER106 ASN107 ALA108 ASP109 B: PRO773 VAL774 PHE777 PRO779 ASN781 TRP782 LEU783 TRP784 ARG785 GLU787 GLN793 ILE794 LEU795 THR796 LEU797 TRP798 LEU799 PRO800 ASP801 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
